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KMID : 0903520010440040211
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2001 Volume.44 No. 4 p.211 ~ p.214
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Effect of Transcriptional Terminator Sequences on Recombinant Protein Expression from Drosophila melanogaster S2 Cell
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Abstract
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Utilizing the foreign gene expression system of the Drosophila melanogaster Schneider line 2(S2) cell, the degree of transient protein and mRNA expression was examined with different terminators. In the case of transient expression, the expression level of green fluorescent protein(GFP) was the highest when the transfection agent was eliminated and then cultivated for 36 to 48 hr. The terminators(SV40 p(A), SV40 small T-antigen and human gastrin 3´ UTR) of the expression vector system were each cloned with endostatin; thereafter, the expression levels of the endostatin and its mRNA were compared. When the expression levels of endostatin were compared 36 hr after transfection, the SV40 p(A) terminator showed the highest expression level of endostatin and its mRNA.
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